Liposomes are prepared by thin film hydration (TFH) method; is very feasible and non-tedious techniques as compared to others. Budesonide and Formoterol liposomes prepared by TFH method and stored in amber colored vial, filled with nitrogen gas and stored in refrigerator till further use. Prepared liposomes were characterized by liposome size, zeta potential and percentage drug entrapment. Liposome size of budesonide and formetrol were 84.8 nm and 87.6 nm respectively. The zeta potential were also minimized i.e. -37 mV for budesonide and 5.21 mV for formetrol liposome. The process parameters like chloroform: methanol ratio, time for solvent evaporation, speed of rotation, volume of hydration, hydration time, number of sonication cycles and vacuum applied were optimized. The organic solvent system chloroform: methanol (4:1) used for preparation of budesonide liposome and chloroform: methanol (2:1) was used for preparation of formetrol liposome. For budesonide: increase in the lipid proportion relative to drug led to the increase in the drug entrapment from 28.51 % to 69.22 %. For formetrol: Increase in the lipid proportion relative to drug led to the increase in the drug entrapment from 22.456 % to 75.02 %. Prepared liposomes of selected drugs were separated by controlled centrifugation at low speed technique was selected for further development of liposomal DPIs.
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